Extraction of Tannic Acid from Kenaf Bast Fibre using Ultrasonic Assisted Extraction

Authors

  • M. Mohamad Universiti Malaysia Kelantan, Faculty of Bioengineering and Technology, Jeli Campus, Kelantan, Malaysia https://orcid.org/0000-0003-1376-5232
  • H.A. Ibrahim Universiti Malaysia Kelantan, Faculty of Bioengineering and Technology, Jeli Campus, Kelantan, Malaysia
  • M.F.M. Nasir National Kenaf and Tobacco Board, Kubang Kerian, Kelantan, Malaysia
  • N.A. Mohidem Universiti Putra Malaysia, Faculty of Engineering Selangor, Department of Biological and Agricultural Engineering, Malaysia https://orcid.org/0000-0001-9892-4823
  • N.F. Shoparwe Universiti Malaysia Kelantan, Faculty of Bioengineering and Technology, Jeli Campus, Kelantan, Malaysia
  • P.T. Teo Universiti Malaysia Kelantan, Faculty of Bioengineering and Technology, Jeli Campus, Kelantan, Malaysia https://orcid.org/0000-0002-2012-5981
  • M.N. Masri Universiti Malaysia Kelantan, Faculty of Bioengineering and Technology, Jeli Campus, Kelantan, Malaysia https://orcid.org/0000-0003-2577-6756
  • A.R. Irfan Universiti Malaysia Perlis, Center of Excellence Geopolymer and Green Technology (CEGeoGTech), Perlis, Malaysia https://orcid.org/0000-0002-6606-9874

DOI:

https://doi.org/10.24425/amm.2022.139703

Abstract

Tannic acid or tannin, type of phenolic compound contains in kenaf bast fibre. Conventional extraction has certain limitations in terms of time, energy, and solvent consumption. Ultrasound assisted extraction (UAE) can extract bioactive components in shorter time, low temperature, with lesser energy and solvent requirement. UAE as alternative extraction technique is better equipped to retain the functionality of the bioactive compounds. In this study, the conditions for ultrasound assisted extraction (UAE) of tannic acid from kenaf bast fibre by assessing the effect of sonication time and different duty cycles were optimized. The use of ultrasound to extract tannic acid from kenaf bast fiber was evaluated. Ultrasound-assisted extraction (UAE) was carried out using ethanol as solvent to intensify the extraction efficacy. Phytochemical screening was conducted to identify the presence of tannic acid in extracts. The extracts then were analyzed using High-Performance Liquid Chromatography (HPLC) and Scanning Electron Microscopy (SEM). It was found that 0.2429 mg/mL of tannic acid was obtained under the extraction conditions of extraction temperature of 40℃, sonication time of 20 minutes and duty cycle of 50%. From SEM analysis, it was found that the raw sample demonstrated rough surface and no porous but kenaf bast fibre display smoother surface with less impurities and few pores appeared after the extraction process using UAE. These results indicate that ultrasound-assisted extraction is an efficient method for extracting tannic acid from kenaf bast fibre with the advantages of lower extraction time and higher extraction yield.

Published

2022-08-26

How to Cite

Mohamad, M., et al. “Extraction of Tannic Acid from Kenaf Bast Fibre Using Ultrasonic Assisted Extraction”. Archives of Metallurgy and Materials, vol. 67, no. 3, Aug. 2022, doi:10.24425/amm.2022.139703.

Issue

Vol. 67 No. 3 (2022)

Section

Articles