First report of Lactococcus garvieae from bovine mastitis in Türkiye characterizing some adhesion-related virulence genes and antimicrobial resistance profiles
DOI:
https://doi.org/10.24425/pjvs.2026.1278Abstract
This research explored the distribution of adhesion-associated virulence determinants and analyzed the antimicrobial susceptibility characteristics of Lactococcus garvieae isolates recovered from bovine mastitis milk samples. Samples were obtained from dairy cows diagnosed with mastitis between 2020 and 2025 and were subsequently sent to the Microbiology Laboratory at the Bornova Veterinary Control Institute (Türkiye) for bacteriological analysis. Polymerase chain reaction (PCR) was performed to detect the presence of adhesion-associated virulence genes, including pavA, psaA, LP1-P4, AC1, AC2 and AF in the L. garvieae strains. Antibiotic susceptibility of the isolates was examined using the disk diffusion method, and multidrug-resistant strains that were resistant to at least three antimicrobial classes were identified. Molecular analyses revealed that pavA, psaA, LP3, AC1 and AC2 genes were present in all isolates (100%). The AF, LP1, and LP4 genes were each detected in two isolates (20%), while LP2 was identified in only one isolate (10%). According to antibiotic susceptibility results, all isolates were susceptible to florfenicol and ampicillin (100%). The highest resistance rate was observed against ciprofloxacin (90%), followed by oxytetracycline and sulfamethoxazole/trimethoprim (80%), penicillin and enrofloxacin (70%), spectinomycin (60%), and erythromycin (40%). Multidrug resistance was detected in nine of the ten isolates (90%), whereas one isolate was resistant only to β-lactams and fluoroquinolones. This study provides the first data on the virulence characteristics of L. garvieae strains isolated from bovine mastitis milk samples in Türkiye. The findings demonstrate that these isolates harbor adhesion-related virulence genes and exhibit a high prevalence of multidrug resistance. These results highlight the pathogenic potential of L. garvieae in bovine mastitis; however, further studies are needed to better elucidate its pathogenic mechanisms, genetic diversity, and resistance traits.
References
Balta I, Simiz FD, Stef D, Pet I, Dumitrescu G, Iancu T, Cretescu I, Corcionivoschi N, Stef L (2025) A new strategy to prevent emerging Lactococcus garvieae infections by using organic acids as antimicrobials in vitro and ex vivo. Int J Mol Sci 26: 3423.
Carvalho MG, Vianni MC, Elliott JA, Reeves M, Facklam RR, Teixeira LM (1997) Molecular analysis of Lactococcus garvieae and Enterococcus gallinarum isolated from water buffalos with subclinical mastitis. Adv Exp Med Biol 418: 401-404.
De Oliveira RP, Aragão BB, de Melo RP, da Silva DM, de Carvalho RG, Juliano MA, Farias MP, de Lira NS, Mota RA (2022) Bovine mastitis in northeastern Brazil: occurrence of emergent bacteria and their phenotypic and genotypic profile of antimicrobial resistance. Comp Immunol Microbiol Infect Dis 85: 101802.
Devriese LA, Hommez J, Laevens H, Pot B, Vandamme P, Haesebrouck F (1999) Identification of aesculin-hydrolyzing streptococci, lactococci, aerococci and enterococci from subclinical intramammary infections in dairy cows. Vet Microbiol 70: 87-94.
Döpfer DD, Almeida RA, Lam TJ, Nederbragt H, Oliver SP, Gaastra W (2000) Adhesion and invasion of Escherichia coli from single and recurrent clinical cases of bovine mastitis in vitro. Vet Microbiol 74: 331-343.
Eraclio G, Ricci G, Quattrini M, Moroni P, Fortina MG (2018) Detection of virulence-related genes in Lactococcus garvieae and their expression in response to different conditions. Folia Microbiol 63: 291-298.
Feito J, Araújo C, Gómez-Sala B, Contente D, Campanero C, Arbulu S, Saralegui C, Peña N, Muñoz-Atienza E, Borrero J, del Campo R, Hernández P, Cintas LM (2022) Antimicrobial activity, molecular typing and in vitro safety assessment of Lactococcus garvieae isolates from healthy cultured rainbow trout (Oncorhynchus mykiss, Walbaum) and rearing environment. LWT 162: 113496.
Francés-Cuesta C, Ansari I, Fernández-Garáyzabal JF, Gibello A, González-Candelas F (2022) Comparative genomics and evolutionary analysis of Lactococcus garvieae isolated from human endocarditis. Microb Genom 8: 000771.
Funke G, Funke-Kissling P (2005) Performance of the new VITEK 2 GP card for identification of medically relevant gram-positive cocci in a routine clinical laboratory. J Clin Microbiol 43: 84-88.
Gibello A, Galán-Sánchez F, Blanco MM, Rodríguez-Iglesias M, Domínguez L, Fernández-Garayzábal JF (2016) The zoonotic potential of Lactococcus garvieae: an overview on microbiology, epidemiology, virulence factors and relationship with its presence in foods. Res Vet Sci 109: 59-70.
Hadimli HH, Sayın Z, Erganiş O, Kav K (2013) Identification and antibiotic susceptibility of catalase negative Gram positive cocci isolated from dairy cows with subclinical mastitis. Eurasian J Vet Sci 29: 153-158.
Hensen SM, Pavičić MJ, Lohuis JA, Poutrel B (2000) Use of bovine primary mammary epithelial cells for the comparison of adherence and invasion ability of Staphylococcus aureus strains. J Dairy Sci 83: 418-429.
Holmes AR, McNab R, Millsap KW, Rohde M, Hammerschmidt S, Mawdsley JL, Jenkinson HF (2001) The pavA gene of Streptococcus pneumoniae encodes a fibronectin-binding protein essential for virulence. Mol Microbiol 41: 1395-1408.
Keane OM (2019) Symposium review: intramammary infections – major pathogens and strain-associated complexity. J Dairy Sci 102: 4713-4726.
Kerro Dego O, van Dijk JE, Nederbragt H (2002) Factors involved in the early pathogenesis of bovine Staphylococcus aureus mastitis with emphasis on bacterial adhesion and invasion. A review. Vet Q 24: 181-198.
Krukowski H, Lisowski A, Puacz E, Chabuz W, Targońska-Karasek M (2025) First report of the isolation and molecular characterisation of Lactococcus garvieae in dairy cattle in Poland. J Vet Res 69: 213-218.
Lin Y, Han J, Barkema HW, Wang Y, Gao J, Kastelic JP, Han B, Qin S, Deng Z (2023) Comparative genomic analyses of Lactococcus garvieae isolated from bovine mastitis in China. Microbiol Spectr 11: e02995-22.
López-Campos G, Aguado-Urda M, Blanco MM, Gibello A, Cutuli MT, López-Alonso V, Martín-Sánchez F, Fernández-Garayzábal JF (2015) Lactococcus garvieae: a small bacteria and a big data world. Health Inf Sci Syst 3: S5.
Maki T, Hirono I, Kondo H, Aoki T (2008) Drug resistance mechanism of the fish-pathogenic bacterium Lactococcus garvieae. J Fish Dis 31: 461-468.
Miller RA, Harbottle H (2018) Antimicrobial drug resistance in fish pathogens. Microbiol Spectr 6: ARBA-0017-2017.
Miyauchi E, Toh H, Nakano A, Tanabe S, Morita H (2012) Comparative genomic analysis of Lactococcus garvieae strains isolated from different sources reveals candidate virulence genes. Int J Microbiol 2012: 728276.
Morita H, Toh H, Oshima K, Yoshizaki M, Kawanishi M, Nakaya K, Suzuki T, Miyauchi E, Ishii Y, Tanabe S, Murakami M, Hattori M (2011) Complete genome sequence and comparative analysis of the fish pathogen Lactococcus garvieae. PLoS One 6: e23184.
Ötkün S, Oğuz AN, Yıldırım F, Tel Y (2024) Identification of Enterococcus spp. and Lactococcus spp. strains isolated from bovine mastitis by MALDI-TOF MS and evaluation of antimicrobial resistance profiles. J Res Vet Med 43: 8-14.
Öztürk RÇ, Ustaoglu D, Ture M, Bondavalli F, Colussi S, Pastorino P, Vela AI, Kotzamanidis C, Fernandez-Garayzábal JF, Bitchava K, Terzi Y, Volpatti D, Altinok I (2024) Epidemiological cutoff values and genetic antimicrobial resistance of Lactococcus garvieae and L. petauri. Aquaculture 593: 741340.
Rodrigues MX, Lima SF, Higgins CH, Canniatti-Brazaca SG, Bicalho RC (2016) The Lactococcus genus as a potential emerging mastitis pathogen group: a report on an outbreak investigation. J Dairy Sci 99: 9864-9874.
Rowe SM, Vasquez AK, Godden SM, Nydam DV, Royster E, Timmerman J, Boyle M (2021) Evaluation of 4 predictive algorithms for intramammary infection status in latelactation cows. J Dairy Sci 104: 11035-11046.
Scillieri Smith JC, Moroni P, Santisteban CG, Rauch BJ, Ospina PA, Nydam DV (2020) Distribution of Lactococcus spp. in New York State dairy farms and the association of somatic cell count resolution and bacteriological cure in clinical mastitis samples. J Dairy Sci 103: 1785-1794.
Sezgin SS, Yılmaz M, Arslan T, Kubilay A (2023) Current antibiotic sensitivity of Lactococcus garvieae in rainbow trout (Oncorhynchus mykiss) farms from Southwestern Turkey. J Agric Sci 29: 630-642.
Salogni C, Bertasio C, Accini A, Gibelli LR, Pigoli C, Susini F, Podavini E, Scali F, Varisco G, Alborali GL (2024) The characterisation of Lactococcus garvieae isolated in an outbreak of septicaemic disease in farmed sea bass (Dicentrarchus labrax, Linnaues 1758) in Italy. Pathogens 13: 49.
Saticioglu IB, Onuk EE, Ay H, Ajmi N, Demirbas E, Altun S (2023) Phenotypic and molecular differentiation of Lactococcus garvieae and Lactococcus petauri isolated from trout. Aquaculture 577: 739933.
Teker T, Albayrak G, Akayli T, Ürkü Ç (2020) Screening of lactococcal adhesion genes and two pneumococcal genes as genetic determinants of virulence in Lactococcus garvieae strains. Genet Aquat Organ 4: 61-67.
Türe M, Altınok I (2016) Detection of putative virulence genes of Lactococcus garvieae. Dis Aquat Org 119: 59-66.
Tejedor JL, Vela AI, Gibello A, Casamayor A, Domínguez L, Fernández-Garayzábal JF (2011) A genetic comparison of pig, cow and trout isolates of Lactococcus garvieae by PFGE analysis. Lett Appl Microbiol 53: 614-619.
Thrusfield M, Christley R, Brown H, Diggle PJ, French N, Howe K, Kelly L, O’Connor A, Sargeant J, Wood H (2018) Veterinary epidemiology. 4th ed., John Wiley & Sons, Chichester.
Vela AI, Vázquez J, Gibello A, Blanco MM, Moreno MA, Liébana P, Albendea C, Alcalá B, Mendez A, Domínguez L, Fernández-Garayzábal JF (2000) Phenotypic and genetic characterization of Lactococcus garvieae isolated in Spain from lactococcosis outbreaks and comparison with isolates of other countries and sources. J Clin Microbiol 38: 3791-3795.
Wu F, Du T, Jiang X, Liu S, Cheng Y, Zhang Z, Miao W, Wang T (2024) Lactococcus garvieae exerts a critical role in inducing inflammation in dairy mastitis by triggering NLRP3 inflammasome-mediated pyroptosis in MAC-T cells. World J Microbiol Biotechnol 40: 132.
Xie X, Pan Z, Yu Y, Yu L, Wu F, Dong J, Wang T, Li L (2023) Prevalence, virulence, and antibiotics gene profiles in Lactococcus garvieae isolated from cows with clinical mastitis in China. Microorganisms 11: 379.
Zlotkin A, Eldar A, Ghittino C, Bercovier H (1998) Identification of Lactococcus garvieae by PCR. J Clin Microbiol 36: 983-985
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