Evaluation of two commercial agarose gel kits for estimating IgG concentrations in neonatal calves: A pilot study

Authors

  • A. Kawamura College of Life, Environment, and Advanced Sciences, Osaka Prefecture University, 1-58, Rinku-ourai-kita, Izumisano-shi, Osaka 598-8531, Japan
  • N. Yamagishi College of Life, Environment, and Advanced Sciences, Osaka Prefecture University, 1-58, Rinku-ourai-kita, Izumisano-shi, Osaka 598-8531, Japan; Graduate School of Veterinary Science, Osaka Metropolitan University, 1-58, Rinku-ourai-kita, Izumisano-shi, Osaka 598-8531, Japan; Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan
  • S. Kim College of Life, Environment, and Advanced Sciences, Osaka Prefecture University, 1-58, Rinku-ourai-kita, Izumisano-shi, Osaka 598-8531, Japan; Graduate School of Veterinary Science, Osaka Metropolitan University, 1-58, Rinku-ourai-kita, Izumisano-shi, Osaka 598-8531, Japan
  • S. Tsuchiaka College of Life, Environment, and Advanced Sciences, Osaka Prefecture University, 1-58, Rinku-ourai-kita, Izumisano-shi, Osaka 598-8531, Japan; Graduate School of Veterinary Science, Osaka Metropolitan University, 1-58, Rinku-ourai-kita, Izumisano-shi, Osaka 598-8531, Japan
  • S. Ishikawa College of Life, Environment, and Advanced Sciences, Osaka Prefecture University, 1-58, Rinku-ourai-kita, Izumisano-shi, Osaka 598-8531, Japan; Graduate School of Veterinary Science, Osaka Metropolitan University, 1-58, Rinku-ourai-kita, Izumisano-shi, Osaka 598-8531, Japan

DOI:

https://doi.org/10.24425/pjvs.2026.3612

Keywords

agarose gel electrophoresis (AGE), β2- and γ-globulins, immunoglobulin G (IgG), neonatal calf, passive transfer of immunity

Abstract

This study characterized agarose gel electrophoresis (AGE) profiles of calf serum and evaluated the accuracy of two commercial AGE kits (J711 and J718) for estimating immunoglobulin G (IgG) concentrations. Samples were collected from 10 calves at birth (Day 0), 10-12 h later (Day 0.5), and 2 days after colostrum ingestion (Day 2). AGE was performed with both kits, and IgG concentrations were measured via ELISA. After colostrum ingestion, β2 globulin (Glb) and γGlb fractions merged electrophoretically and were analyzed together as β2γGlb. On Day 0, γglobulin was nearly absent, confirming no transplacental Ig transfer. By Day 2, β2γGlb had markedly increased. Linear mixed-effects models showed that β2γGlb was a strong predictor of IgG levels, with marginal R2 values of 0.960 (J711) and 0.958 (J718). The results suggest that the AGE kits have potential as a practical alternative for evaluating passive immunity in calves.

References

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Published

2026-09-17

How to Cite

Kawamura, A., et al. “Evaluation of Two Commercial Agarose Gel Kits for Estimating IgG Concentrations in Neonatal Calves: A Pilot Study”. Polish Journal of Veterinary Sciences, vol. 29, no. 3, Sept. 2026, pp. 469-72, doi:10.24425/pjvs.2026.3612.

Issue

Vol. 29 No. 3 (2026)

Section

Short communication

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Copyright (c) 2026 Polish Journal of Veterinary Sciences

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