Development of a SYBR Green I real-time PCR assay for detection of novel porcine parvovirus 7

Authors

  • Y.D. Li Municipal Key Laboratory of Virology, Ningbo Municipal Center for Disease Control and Prevention, Ningbo 315010, PR China
  • Z.D. Yu Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, PR China
  • C.X. Bai Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, PR China
  • D. Zhang Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, PR China
  • P. Sun Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, PR China
  • M.L. Peng Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, PR China
  • H. Liu Anhui Animal Diseases Prevention and Control Center and Key Laboratory of Veterinary Pathobiology and Disease Prevention and Control of Anhui Province, Hefei 230091, PR China
  • J. Wang Animal Husbandry Base Teaching and Research Section, College of Animal Science and Technology, Hebei North University, Hebei 075000, PR China
  • Y. Wang Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, PR China

DOI:

https://doi.org/10.24425/pjvs.2021.136791

Abstract

In this study, we developed a SYBR Green I real-time PCR method for the rapid and sensitive detection of novel porcine parvovirus 7 (PPV7). Specific primers were designed based on the highly conserved region within the Capsid gene of PPV7. The established method was 1,000 times more sensitive than the conventional PCR method and had a detection limit of 35.6 copies. This method was specific and had no cross-reactions with PCV2, PCV3, PRV, PEDV, PPV1, and PPV6. Experiments testing the intra and interassay precision demonstrated a high reproducibility. Testing the newly established method with 200 clinical samples revealed a detection rate up to 17.5% higher than that of the conventional PCR assay. The established method could provide technical support for clinical diagnosis and epidemiological investigation of PPV7.

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Published

2021-03-30

How to Cite

Li, Y.D., et al. “Development of a SYBR Green I Real-Time PCR Assay for Detection of Novel Porcine Parvovirus 7”. Polish Journal of Veterinary Sciences, vol. 24, no. 1, Mar. 2021, pp. 43-49, doi:10.24425/pjvs.2021.136791.

Issue

Vol. 24 No. 1 (2021)

Section

Article