Development of an indirect enzyme-linked immunosorbent assay for the detection of novel chicken orthoreovirus

Authors

  • H. Liu College of Veterinary Medicine, Shandong Agricultural University, No.61 Daizong Street, Tai’an 271018, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, No.61 Daizong Street, Tai’an 271018, China; Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Shandong Agricultural University, No.61 Daizong Street, Tai’an 271018, China
  • Z. Wei College of Veterinary Medicine, Shandong Agricultural University, No.61 Daizong Street, Tai’an 271018, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, No.61 Daizong Street, Tai’an 271018, China; Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Shandong Agricultural University, No.61 Daizong Street, Tai’an 271018, China
  • J. Yang College of Veterinary Medicine, Shandong Agricultural University, No.61 Daizong Street, Tai’an 271018, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, No.61 Daizong Street, Tai’an 271018, China; Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Shandong Agricultural University, No.61 Daizong Street, Tai’an 271018, China
  • Y. Wang College of Veterinary Medicine, Shandong Agricultural University, No.61 Daizong Street, Tai’an 271018, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, No.61 Daizong Street, Tai’an 271018, China; Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Shandong Agricultural University, No.61 Daizong Street, Tai’an 271018, China
  • J. Hu College of Veterinary Medicine, Shandong Agricultural University, No.61 Daizong Street, Tai’an 271018, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, No.61 Daizong Street, Tai’an 271018, China; Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Shandong Agricultural University, No.61 Daizong Street, Tai’an 271018, China
  • Y. Tang College of Veterinary Medicine, Shandong Agricultural University, No.61 Daizong Street, Tai’an 271018, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, No.61 Daizong Street, Tai’an 271018, China; Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Shandong Agricultural University, No.61 Daizong Street, Tai’an 271018, China
  • Y. Diao College of Veterinary Medicine, Shandong Agricultural University, No.61 Daizong Street, Tai’an 271018, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, No.61 Daizong Street, Tai’an 271018, China; Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Shandong Agricultural University, No.61 Daizong Street, Tai’an 271018, China

DOI:

https://doi.org/10.24425/pjvs.2022.140847

Abstract

A novel avian orthoreovirus (N-ARV) variant characterized with obvious arthritis and synovial inflammation, was isolated from Shandong, China in May 2016. It caused chicken poor growth and enormous economic losses to the poultry industry of China. However, there are few effective methods for detecting the antibody levels of N-ARV. In this study, a viral structural protein σC was expressed using the prokaryotic expression vector pET32a (+). The target protein was obtained by inducing for 6 hours at an IPTG concentration of 0.6mM. The optimal dilution of the coating antigen and serum antibody were determined to be 1000 fold and 10 fold respectively. A specificity test showed that there was no positive reactivity between N-ARV and other pathogens, and when the positive serum was diluted 100 times detection results were still checkable. The repeatability of this method was determined by the inter assay and intra assay tests with variability ranging from 4.85% to 7.93%. In conclusion, this indirect enzyme linked immunosor bent assay (ELISA) will be useful for large-scale serological surveys and monitoring antibody levels in N-ARV infection.

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Published

2022-03-30

How to Cite

Liu, H., et al. “Development of an Indirect Enzyme-Linked Immunosorbent Assay for the Detection of Novel Chicken Orthoreovirus”. Polish Journal of Veterinary Sciences, vol. 25, no. 1, Mar. 2022, pp. 109-18, doi:10.24425/pjvs.2022.140847.

Issue

Vol. 25 No. 1 (2022)

Section

Article