Environmental risk assessment of antibiotic residues in cattle slurry used as fertiliser
DOI:
https://doi.org/10.24425/jwld.2025.155308Abstract
The aim of the study was to assess the environmental risk caused by active antibiotic substances present in cattle slurry from ecological farms, used for fertilisation purposes. In order to assess the environmental risk, studies were carried out on farms located in the cleanest region of north-eastern Poland. The study focused on substances most commonly found in veterinary drugs: tetracycline, bacitracin, kanamycin, dihydrostreptomycin, benzylpenicillin, cefquinome, and cephalexin. In the analysed cattle slurry, only tetracycline, benzylpenicillin, and cephalexin were detected in the analysed slurry. Their concentrations were respectively 1.53-15.1, 0.048-0.27, and nd-1.11 ng∙g-1. Other tested antibiotics, such as cefquinome and dihydrostreptomycin, were detected in the samples of cattle slurry at concentrations below the LOQ. Bacitracin and kanamycin were not detected in any of the samples. The environmental risk assessment, based on the risk quotient (RQ) values calculated for tetracycline and benzylpenicillin, indicated that the potential risk to soil was well below the level of concern (LoC). The risk assessment for cephalexin could not be performed due to the lack of sufficient data. The analysis indicates that the risk to the soil compartment from the application of cattle slurry originating from organic farms is below the level of concern. However, due to data gaps in the available literature, a comprehensive analysis could not be completed.References
Balizs, G. and Hewitt, A. (2003) “Determination of veterinary drug residues by liquid chromatography and tandem mass spectrometry,” Analytica Chimica Acta, 492, pp. 105–131. Available at: https://doi.org/10.1016/S0003-2670(03)00890-0.
Boxall, A.B.A. et al. (2004) “Veterinary medicines in the environment,” in Reviews of environmental contamination and toxicology. New York: Springer, pp. 1–91. Available at: https://doi.org/10.1007/0-387-21729-0_1.
Chowdhury, S. et al. (2021) “Antibiotic usage and resistance in food animal production: What have we learned from Bangladesh?,” Antibiotics, 10, 1032. Available at: https://doi.org/10.3390/antibiotics10091032.
Christian T. et al. (2003) “Determination of antibiotic residues in manure, soil, and surface waters,” Acta Hydrochimica et Hydrobiologica, 31, pp. 36–44. Available at: https://doi.org/10.1002/aheh.200390014.
Ciucă, V.C. et al. (2023) “Rapid environmental impact assessment of penicillin G in a veterinary product using an original software method and monitoring by SPE-online-UHPLC-MS/MS,” Molecules, 28, 6227. Available at: https://doi.org/10.3390/molecules28176227.
Cycoń, M., Mrozik, A. and Piotrowska-Seget, Z. (2019) “Antibiotics in the soil environment-degradation and their impact on microbial activity and diversity,” Frontiers in Microbiology, 10, 338. Available at: https://doi.org/10.3389/fmicb.2019.00338.
Czarny, J. et al. (2024) “Determination of 465 psychoactive substances, drugs and their metabolites in urine by LC-MS/MS,” Analytical Methods, 16, pp. 5426–5432. Available at: https://doi.org/10.1039/D4AY00777H.
Dolliver, H., Gupta, S. and Noll, S. (2008) “Antibiotic degradation during manure composting,” Journal of Environmental Quality, 37, pp. 1245–1253. Available at: https://doi.org/10.2134/jeq2007.0399.
Dongen van, K.C.W. et al. (2024) “Safety and transfer of veterinary drugs from substrate to black soldier fly larvae,” Animal, 18, 101214. Available at: https://doi.org/10.1016/j.animal.2024.101214.
Emes, E., Kagambèga, A. and Dione, M. (2024) “Determinants of animal disease and nontherapeutic antibiotic use on smallholder livestock farms,” Frontiers in Veterinary Science, 10, 1258214. Available at: https://doi.org/10.3389/fvets.2023.1258214.
Fang, L. et al. (2023) “A comprehensive and global evaluation of residual antibiotics in agricultural soils: Accumulation, potential ecological risks, and attenuation strategies,” Ecotoxicology and Environmental Safety, 262, 115175. Available at: https://doi.org/10.1016/j.ecoenv.2023.115175.
Gaballah, M.S. et al. (2021) “A review targeting veterinary antibiotics removal from livestock manure management systems and future outlook,” Bioresource Technology, 333, 125069. Available at: https://doi.org/10.1016/j.biortech.2021.125069.
GUS (2024) Rocznik statystyczny Rzeczypospolitej Polskiej 2023 [Statistical yearbook of the Republic of Poland 2023]. Warszawa: Główny Urząd Statystyczny. Available at: https://stat.gov.pl/obszary-tematyczne/roczniki-statystyczne/roczniki-statystyczne/rocznik-statystyczny-rzeczypospolitej-polskiej-2023,2,23.html (Accessed: January 15, 2025).
Halling-Sørensen, B. et al. (1998) “Occurrence, fate, and effects of pharmaceutical substances in the environment – A review,” Chemosphere, 36, pp. 357–393. Available at: https://doi.org/10.1016/S0045-6535(97)00354-8.
Hamscher, G. et al. (2005) “Different behavior of tetracyclines and sulfonamides in sandy soils after repeated fertilization with liquid manure,” Environmental Toxicology and Chemistry, 24, pp. 861– 868. Available at: https://doi.org/10.1897/04-182R.1.
Hu, X., Zhou, Q. and Luo, Y. (2010) “Occurrence and source analysis of typical veterinary antibiotics in manure, soil, vegetables and groundwater from organic vegetable bases, northern China,” Environmental Pollution, 158, pp. 2992–2998. Available at: https://doi.org/10.1016/j.envpol.2010.05.023.
Huygens, J. et al. (2022) “Impact of fertilization with pig or calf slurry on antibiotic residues and resistance genes in the soil,” Science of the Total Environment, 822, 153518. Available at: https://doi.org/10.1016/j.scitotenv.2022.153518.
Joy, S.R. et al. (2013) “Fate and transport of antimicrobials and antimicrobial resistance genes in soil and runoff following land application of swine manure slurry,” Environmental Science & Technology, 47, pp. 12081−12088. Available at: https://doi.org/10.1021/es4026358.
Karcı, A. and Balcıoğlu, I.A. (2009) “Investigation of the tetracycline, sulfonamide, and fluoroquinolone antimicrobial compounds in animal manure and agricultural soils in Turkey,” Science of the Total Environment, 407, pp. 4652–4664. Available at: https://doi.org/10.1016/j.scitotenv.2009.04.047.
Kemper, N. et al. (2008) “Analysis of antibiotic residues in liquid manure and leachate of dairy farms in northern Germany,” Agricultural Water Management, 95, pp. 1288–1292. Available at: https://doi.org/10.1016/J.AGWAT.2008.05.008.
Kumar, K. et al. (2005) “Antibiotic use in agriculture and its impact on the terrestrial environment,” Advances in Agronomy, 87, pp. 1–54. Available at: https://doi.org/10.1016/S0065-2113(05)87001-4.
Lihong, C. et al. (2021) “Predicted non-effect concentration and risk assessment of typical tetracycline antibiotics,” Asian Journal of Ecotoxicology, 16(5), pp. 334–346. Available at: https://doi.org/10.7524/AJE.1673-5897.20210602001.
Lu, X. et al. (2014) “Long-term effect of the antibiotic cefalexin on methane production during waste activated sludge anaerobic digestion,” Bioresource Technology, 169, pp. 644–651. Available at: https://doi.org/10.1016/j.biortech.2014.07.056.
Martínez-Carballo, E. et al. (2007) “Environmental monitoring study of selected veterinary antibiotics in animal manure and soils in Austria,” Environmental Pollution, 148, pp. 570–579. Available at: https://doi.org/10.1016/j.envpol.2006.11.035.
Massaccesi, L. et al. (2024) “Impact of soil fertilization with pig slurry on antibiotic residues and resistance genes: A longitudinal study,” Antibiotics, 13, 486. Available at: https://doi.org/10.3390/anti-biotics13060486.
Meyer, A.M. et al. (2021) “Chemical food safety hazards of insects reared for food and feed,” Journal of Insects as Food and Feed, 7, pp. 823–831. Available at: https://doi.org/10.3920/JIFF2020.0085.
Miciński, J. et al. (2015) “Hazards to humans and animals associated with antibiotic misuse,” Journal of Elementology, 20(4), pp. 1077– 1086. Available at: https://doi.org/10.5601/jelem.2015.20.2.902.
Muscat, A. et al. (2021) “Principles, drivers and opportunities of a circular bioeconomy,” Nature Food, 2, pp. 561–566. Available at: https://doi.org/10.1038/s43016-021-00340-7.
Mulchandani, R. et al. (2023) “Global trends in antimicrobial use in food-producing animals: 2020 to 2030,” PLOS Global Public Health, 3(2), e0001305. Available at: https://doi.org/10.1371/journal.pgph.0001305.
Nightingale, J. et al. (2022) “Assessing the influence of pig slurry pH on the degradation of selected antibiotic compounds,” Chemosphere, 290, 133191. Available at: https://doi.org/10.1016/j.chemosphere.2021.133191.
Oliver, J.P. et al. (2020) “Invited review: Fate of antibiotic residues, antibiotic-resistant bacteria, and antibiotic resistance genes in US dairy manure management systems,” Journal of Dairy Science, 103, 2, pp. 1051–1071. Available at: https://doi.org/10.3168/jds.2019-16778.
Okon, A.J. et al. (2023) “Knowledge, practices, and antibiotics use patterns among animal production farmers in Calabar Metropolis,” International Journal of Public Health Science (IJPHS), 12(4), pp. 1647–1655. Available at: https://doi.org/10.11591/ijphs.v12i4.23266.
Patyra, E. et al. (2019) “Występowanie tetracyklin w paszach – przyczyny i skutki [Use of tetracyclines in feeds: Causes and consequences],” Medycyna Weterynaryjna, 75(5), pp. 280–286. Available at: http://dx.doi.org/10.21521/mw.6152.
PN-B-12098:1997 – Road drainage – Requirements and testing. Warszawa: Polski Komitet Normalizacyjny.
Regulation (2019) “Regulation (EU) 2019/6 of the European Parliament and of the Council of 11 December 2018 on veterinary medicinal products and repealing Directive 2001/82/EC.280–286 (Text with EEA relevance),” Official Journal, 4, 7.1.2019, pp. 43–167. Available at: https://eur-lex.europa.eu/legal-content/EN/TXT/?uri=CELEX:32019R0006 (Accessed: January 15, 2025).
Sacher, F. et al. (2001) “Pharmaceuticals in ground waters. Analytical methods and result of a monitoring program in Baden-Wurttemberg, Germany,” Journal of Chromatography A, 938, pp. 199–210. Available at: https://doi.org/10.1016/S0021-9673(01)01266-3.
Sarmah, K., Meyer, M.T. and Boxall, A.B.A. (2006) “A global perspective on the use, sales, exposure pathways, occurrence, fate and effects of veterinary antibiotics (VAs) in the environment,” Chemosphere, 65, pp. 725–759. Available at: https://doi.org/10.1016/j.chemosphere.2006.03.026.
Spielmeyer, A., Ahlborn, J. and Hamscher, G. (2014) “Simultaneous determination of 14 sulfonamides and tetracyclines in biogas plants by liquid-liquid-extraction and liquid chromatography tandem mass spectrometry,” Analytical and Bioanalytical Chemistry, 406, pp. 2513–2524. Available at: https://doi.org/10.1007/s00216-014-7649-3.
Spielmeyer, A. et al. (2015) “Elimination patterns of worldwide used sulfonamides and tetracyclines during anaerobic fermentation,” Bioresource Technology, 193, pp. 307–314. Available at: https://doi.org/10.1016/j.biortech.2015.06.081.
Widyasari-Mehta, A., Hartung, S. and Kreuzig, R. (2016) “From the application of antibiotics to antibiotic residues in liquid manures and digestates: A screening study in one European center of conventional pig husbandry,” Journal of Environmental Management, 177, pp. 129–137. Available at: https://doi.org/10.1016/j.jenvman.2016.04.012.
Wohde, M. et al. (2016) “Occurrence and transformation of veterinary pharmaceuticals and biocides in manure: a literature review,” Environmental Sciences Europe, 28, 23. Available at: https://doi.org/10.1186/s12302-016-0091-8.
Wolters, B. et al. (2016) “Contaminations of organic fertilizers with antibiotic residues, resistance genes, and mobile genetic elements mirroring antibiotic use in livestock?,” Applied Microbiology and Biotechnology, 100, pp. 9343–9353. Available at: https://doi.org/10.1007/s00253-016-7742-y.
Xu, T. et al. (2024) “Progress in combating antibiotic resistance in animal agriculture,” CyTA – Journal of Food, 22(1), 2330674. Available at: https://doi.org/10.1080/19476337.2024.2330674.
Yang, Z. et al. (2024) “How do different antibiotic residues in manure change soil N2O emissions and soil N-cycling microbial communities?,” Applied Soil Ecology, 202, 105577. Available at: https://doi.org/10.1016/j.apsoil.2024.105577.
Yordanova, R., Platikanova, M. and Hristova, P. (2024) “The use of antibiotics in food animals – a threat to human health,” Journal of IMAB, 30(2), pp. 5495–5499. Available at: https://doi.org/10.5272/jimab.2024302.5495.
Zhao, L., Dong, Y.H. and Wang, H (2010) “Residues of veterinary antibiotics in manures from feedlot livestock in eight provinces of China,” Science of The Total Environment, 408, pp. 1069–1075. Available at: https://doi.org/10.1016/j.scitotenv.2009.11.014
