Bio-efficacy and dynamic distribution of pymetrozine and cyantraniliprole against Aphis gossypii glover, and their residues in cucumber (Cucumis sativus l.) fruits using the QuEChERS method and LC-ESI-MS/MS
DOI:
https://doi.org/10.24425/jppr.2026.158066Abstract
Mixed pesticides are promising alternative approaches for insect pest management and could reduce the risk to the environment. Therefore, their efficiency against pests and their residues in the environment should be investigated. In this study, the efficacy of pymetrozine, cyantraniliprole, and their mixture against Aphis gossypii Glover and their residues in cucumber (Cucumis sativus L.) were studied. The results showed that pymetrozine and cyantraniliprole alone or in combination were more effective than acetamiprid insecticide for reducing A. gosspyii populations up to 15 days following application. Additionally, a residual analytical method for insecticide determination in cucumber fruits, leaves, and soil was optimized and validated. The tested insecticides’ residue extraction was carried out using the QuEChERS method, and the determination was achieved using liquid chromatography- tandem mass spectrometry (LC-MS/MS). The recovery tests were studied at three spiking levels of low concentration (0.01, 0.05, and 0.1 mg · kg–1) with acceptable recovery between 71 and 101% and good precision and expanded uncertainty up to ± 20%. The half-life times of the tested formulations ranged from 8.19 to 10.6 days, while the preharvest intervals (PHI) ranged from 8.8 to 23.8 days.
References
Abd-Alrahman S.H., Kotb G.A.M. 2020. Dissipation kinetics of pymetrozine in Tomato field ecosystem. The Egyptian Journal of Hospital Medicine 81: 2305–2309. DOI: 10.21608/ejhm.2020.131248
Awad M., Hassan N.N., Alfuhaid N.A., Amer A., Salem M.Z.M., Fónagy A., Moustafa M.A.M. 2024. Insecticidal and biochemical impacts with molecular docking analysis of three essential oils against Spodoptera littoralis (Lepidoptera: Noctuidae). Crop Protection 180: 106659. DOI: 10.1016/j.cropro.2024.106659
Ding W., Guo L., Xue Y., Wang M., Li C., Zhang R., Xia X. 2024. Life parameters and physiological reactions of Cotton Aphids Aphis gossypii (Hemiptera: Aphididae) to sublethal concentrations of Afidopyropen. Agronomy 14: 258. DOI: https://doi.org/10.3390/agronomy14020258
Ebert T.A., Cartwright B. 1997. Biology and ecology of Aphis gossypii Glover (Homoptera: aphididae). Southweste Entomologi 22: 116–153.
El-Hefny D.E., Ibrahim E.S., Alfuhaid N.A., Fónagy A., Moustafa M.A.M. 2024 Residual Effect of the Insecticides Flonicamid and Spiromesifen against Aphis craccivora (Hemiptera: Aphididae) and Persistence Dynamics in Faba Bean. Journal of Entomological Science 59: 165–181. DOI: 10.18474/JES2339
El-Shourbagy N.M., Farag S.M., Moustafa M.A.M., Al-Shuraym L.A., Sayed S., Zyaan O.H. 2023. Biochemical and insecticidal efficacy of clove and basil essential oils and two photosensitizers and their combinations on Aphis gossypii Glover (Hemiptera: Aphididae). Bioscience Journal 39: e39100. DOI: 10.14393/BJ-v39n0a2023-69129
Farag A.I., Said S.M., Abd El- Raheem A.M., Swalem N.O., Ataa O.I. 2024. Influence of five host plants on development and life table Parameters of Aphis gossypii Glover (Hemiptera: Aphididae). Egyptian Academic Journal of biological sciences17: 25-32. DOI: 10.21608/eajbsa.2024.353867
Ferguson J.S., Koenig J.P., White S.M., Dunbar D.M., Lawson D.S. 1999. Evaluation of Fulfill® 50WG (pymetrozine) for cotton aphid control in 1998 field trails. Proceedings of the Beltwide Cotton Conference 2: 1016–1019.
Hanafy A.R. 2004. Studies on the most important Cucumber pests in the open field and suitable control programs. Ph.D. thesis, Fac. of Agric. Moshtohor, Benha Branch- Zagazig Univ., Egypt, 279 pp.
Handerson C.F., Tilton E.W. 1955. Tests with acaricides against the brown wheat mite. Journal of Economic Entomology 48: 157–161. DOI: 10.1093/jee/48.2.157
Harrewijn P., Kayser H. 1997. Pymetrozine, a fast-acting and selective inhibitor of aphid feeding. In-situ studies with electronic monitoring of feeding behaviour. Pesticide Science 49: 130–140. DOI: 10.1002/(sici)1096-9063(199702)49:2<130::aid-ps509>3.0.co;2-u
Heilsnis B., Mahas J.B., Conner K., Pandey S., Clark W., Koebernick J., Jacobson A.L. 2023. Characterizing the vector competence of Aphis gossypii, Myzus persicae and Aphis craccivora (Hemiptera: Aphididae) to transmit cotton leafroll dwarf virus to cotton in the United States. Journal of Economic Entomology 116: 719–725. DOI: 10.1093/jee/toad080
Hong J.-H., Lee C.-R., Lim J.-S., Lee K.-S., Kyu L. 2011. Comparison of analytical methods and residue patterns of pymetrozine in Aster scaber. Bulletin of Environmental Contamination and Toxicology 87: 649–652. DOI: 10.1007/s00128-011-0407-8
Kandil M.A., Moustafa M.A.M., Saleh M.A., Ateya I.R. 2023. Dissipation kinetics and degradation products of cyantraniliprole in tomato plants and soil in the open field. Egyptian Journal of Chemistry 66: 483–493. DOI: 10.21608/ejchem.2023.195133.7625
Kim S.E., Kim H.K., Kim G.H. 2024. Sublethal effects of spirotetramat, cyantraniliprole, and pymetrozine on Aphis gossypii (Hemiptera: Aphididae). Insects 15: 247. DOI:10.3390/insects15040247
Koo H.-N., An J.-J., Park S.-E., Kim J.-I., Kim G.-H. 2014. Regional susceptibilities to 12 insecticides of melon and cotton aphid, Aphis gossypii (Hemiptera: Aphididae) and a point mutation associated with imidacloprid resistance. Crop Protection 55: 91–97. DOI: 10.1016/j.cropro.2013.09.010
Krishnan V.N. 2009. DPX-HGW86: Laboratory study of solubility in organic solvents.
Kristinsson H. 1994. Pymetrozine: A new insecticide. In Advances in the Chemistry of Insect Control III, pp. 85-102. Cambridge: The Royal Society of Chemistry.
Magnusson B., Örnemark U. 2014. Eurachem Guide: The Fitness for Purpose of Analytical Methods– A Laboratory Guide to Method Validation and Related Topics, (2nd ed.). https://www.eurachem.org/images/stories/Guides/pdf/MV_guide_2nd_ed_EN.pdf
Malhat F., Abdallah O. 2019. Residue distribution and risk assessment of two macrocyclic lactone insecticides in green onion using micro-liquid-liquid extraction (MLLE) technique coupled with liquid chromatography tandem mass spectrometry. Environmental Monitoring and Assessment 191: 1–10. DOI: 10.1007/s10661-019-7752-1
Mohamed M.A., Homam H.B. 2012. Using some insecticides for controlling cotton and watermelon aphid, Aphis gossypii Glover on cucumber plants referring to the yield. Egyptian J. Agr. Res. 90: 517–527. DOI: 10.21608/ejar.2012.160147
Moustafa M.A.M., Amer A., Al-Shuraym L.A., Ibrahim E.S., El-Hefny D.E., Salem M.Z.M., Sayed S. 2022. Efficacy of chemical and bio-pesticides on cowpea aphid, Aphis craccivora, and their residues on the productivity of fennel plants (Foeniculum vulgare). Journal of King Saud University – Science 34: 101900. DOI: 10.1016/j.jksus.2022.101900
Moustafa M.A.M., El Hefny D.E., Alfuhaid N.A., Helmy R.M.A., El-Said N.A., Ibrahim E.S. 2024b. Effectiveness and biochemical impact of flubendiamide and flonicamid insecticides against Bemisia tabaci (Hemiptera: Aleyrodidae) and residue dissipation in Tomato plants and soil under greenhouse conditions. Journal of Entomological Science 59: 289–310. DOI: 10.18474/JES23-61
Moustafa M.A.M., Osman E.A., Mokbel E.M.S., Fouad E.A. 2024a. Biochemical and molecular characterization of chlorantraniliprole resistance in Spodoptera littoralis (Lepidoptera: Noctuidae). Crop Protection 177: 106533. DOI: 10.1016/j.cropro.2023.106533
Pes M.P., Melo A.A., Stacke R.S., Zanella R.Z., Perini C.R., Silva F.M.A., Guedes J.V.C. 2020. Translocation of chlorantraniliprole and cyantraniliprole applied to corn as seed treatment and foliar spraying to control Spodoptera frugiperda (Lepidoptera: Noctuidae). PLoS ONE 15: e0229151. DOI: 10.1371/journal.pone.0229151
Sattelle D.B., Cordova D., Cheek T.R. 2008. Insect ryanodine receptors: molecular targets for novel pest control chemicals. Invertebrate Neuroscience 8: 107–119. DOI: 10.1007/s10158-008-0076-4
Selby T.P., Lahm G.P., Stevenson T.M., Hughes K.A., Cordova D., Annan I.B., Pahutski T.F. 2013. Discovery of cyantraniliprole, a potent and selective anthranilic diamide ryanodine receptor activator with cross-spectrum insecticidal activity. Bioorganic & medicinal chemistry letters 23: 6341–6345. DOI: 10.1016/j.bmcl.2013.09.076
Somar R.O., Zamani A.A., Alizadeh M. 2019. Joint action toxicity of imidacloprid and pymetrozine on the melon aphid, Aphis gossypii. Crop Protection 124: 104850. DOI: 10.1016/j.cropro.2019.104850
Talebi-Jahromi K. 2007. Pesticide toxicology. University of Tehran Publication, Tehran. 492 pp.
Tomizawa M., Casida J.E. 2005. Neonicotinoid insecticide toxicology: mechanisms of selective action. Annual Review of Pharmacology and Toxicology 45: 247–268. DOI: 10.1146/annurev.pharmtox.45.120403.095930
Ullah F., Gul H., Desneux N., Qu Y., Xiao X., Khattak A.M., Gao X., Song D. 2019. Acetamiprid-induced hormetic effects and vitellogenin gene (Vg) expression in the melon aphid, Aphis gossypii Entomologia Generalis 39: 259-270. DOI: 10.1127/entomologia/2019/0887
Wyss P., Bolinger M. 1997. Translocation of pymetrozine in plants. Pesticide Science 50: 195–202. DOI: 10.1002/(SICI)1096-9063(199707)50:33.0.CO;2-D
Wang K.Y., Guo Q.L., Xia X.M., Wang H.Y., Liu T.X. 2007. Resistance of Aphis gossypii (Homoptera:Aphididae) to selected insecticides on cotton from five cotton production regions in Shandong, China. Journal of Pesticide Science 32: 372–378. DOI: 10.1584/jpestics.G06-51
Zhang J., Kothalawala S., Yu C. 2023. Engineered silica nanomaterials in pesticide delivery: challenges and perspectives. Environmental Pollution 320: 121045. DOI: 10.1016/j.envpol.2023.121045
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2026 Journal of Plant Protection Research
This work is licensed under a Creative Commons Attribution 4.0 International License.
